Bürker chamber

The Bürker makes it possible to count "cells". The Bürker room has 9 large squares (1mm² each) divided by double lines (0.05mm apart) into 16 group squares. The double lines form small squares of 0.0025 mm². The chamber depth is 0.1 mm. The cells are counted in each of the 4 large squares (identified by the triple line and shaded in the figure). At the end of the procedure, the operators calculate the mean of the 4 readings (from 4 large squares) and calculate the cell concentration using a formula.